CTIF is a component of the CBP80 (NCBP1; MIM 600469)/CBP20 (NCBP2; MIM 605133) translation initiation complex that binds cotranscriptionally to the cap end of nascent mRNA. The CBP80/CBP20 complex is involved in a simultaneous editing and translation step that recognizes premature termination codons (PTCs) in mRNAs and directs PTC-containing mRNAs toward nonsense-mediated decay (NMD). On mRNAs without PTCs, the CBP80/CBP20 complex is replaced with cytoplasmic mRNA cap-binding proteins, including EIF4G (MIM 600495), and steady-state translation of the mRNAs resumes in the cytoplasm (Kim et al., 2009 [PubMed 19648179]).[supplied by OMIM, Dec 2009]
Transcription factors with Perturb-seq knockdown data for CTIF. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CTIF upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CTIF, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:48,351,343–48,352,076 | 187.3 kb | Distal (>10kb) Multiome | 63 | |
| chr18:48,356,963–48,357,690 | 181.7 kb | Distal (>10kb) Multiome | 83 | |
| chr18:48,408,735–48,410,949 | 129.9 kb | Distal (>10kb) Multiome | 434 | |
| chr18:48,538,422–48,540,251 | 12 bp | At TSS Multiome | 704 | |
| chr18:48,540,359–48,541,661 | 2.3 kb | Proximal (<10kb) Multiome | 670 | |
| chr18:48,542,873–48,543,072 | 3.8 kb | Proximal (<10kb) | 90 | |
| chr18:48,543,194–48,543,725 | 4.2 kb | Proximal (<10kb) | 216 | |
| chr18:48,545,718–48,546,003 | 6.7 kb | Proximal (<10kb) | 190 | |
| chr18:48,548,060–48,548,298 | 9.0 kb | Proximal (<10kb) | 147 | |
| chr18:48,605,317–48,606,127 | 66.6 kb | Distal (>10kb) Multiome | 428 | |
| chr18:48,776,915–48,777,546 | 238.2 kb | Distal (>10kb) Multiome | 356 | |
| chr18:48,802,644–48,803,302 | 264.0 kb | Distal (>10kb) Multiome | 62 |
Genomic view of the CTIF locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.