This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011]
Transcription factors with Perturb-seq knockdown data for AFDN. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AFDN upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AFDN, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:167,652,499–167,653,099 | 174.1 kb | Distal (>10kb) Multiome | 151 | |
| chr6:167,678,066–167,679,144 | 148.1 kb | Distal (>10kb) Multiome | 370 | |
| chr6:167,744,803–167,745,904 | 81.6 kb | Distal (>10kb) Multiome | 117 | |
| chr6:167,750,915–167,751,671 | 75.7 kb | Distal (>10kb) Multiome | 387 | |
| chr6:167,825,427–167,828,107 | 1.2 kb | Proximal (<10kb) Multiome | 895 | |
| chr6:167,892,666–167,893,136 | 542 bp | At TSS | 86 | |
| chr6:167,893,383–167,893,698 | at TSS | At TSS | 64 | |
| chr6:168,123,195–168,123,950 | 296.8 kb | Distal (>10kb) Multiome | 56 | |
| chr6:168,125,394–168,126,053 | 298.8 kb | Distal (>10kb) Multiome | 50 |
Genomic view of the AFDN locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.