WNK3
WNK lysine deficient protein kinase 3 | PRKWNK3

This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Member of: DE-4 DE-4.14 Developmental clusters: GC3
Biological processes 50 terms
ATP binding (GO:0005524)ATP binding (GO:0005524)DNA damage response (GO:0006974)adherens junction (GO:0005912)adherens junction (GO:0005912)bicellular tight junction (GO:0005923)bicellular tight junction (GO:0005923)cell volume homeostasis (GO:0006884)cell volume homeostasis (GO:0006884)cell volume homeostasis (GO:0006884)cell volume homeostasis (GO:0006884)cellular hyperosmotic response (GO:0071474)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)intracellular signal transduction (GO:0035556)intracellular signal transduction (GO:0035556)maintenance of blood-brain barrier (GO:0035633)membraneless organelle assembly (GO:0140694)molecular condensate scaffold activity (GO:0140693)monoatomic ion homeostasis (GO:0050801)negative regulation of apoptotic process (GO:0043066)negative regulation of pancreatic juice secretion (GO:0090188)negative regulation of protein localization to plasma membrane (GO:1903077)osmosensory signaling pathway (GO:0007231)positive regulation of calcium ion transport (GO:0051928)positive regulation of canonical Wnt signaling pathway (GO:0090263)positive regulation of peptidyl-threonine phosphorylation (GO:0010800)positive regulation of protein localization to plasma membrane (GO:1903078)positive regulation of sodium ion transmembrane transporter activity (GO:2000651)positive regulation of sodium ion transmembrane transporter activity (GO:2000651)positive regulation of sodium ion transport (GO:0010765)protein binding (GO:0005515)protein kinase activity (GO:0004672)protein kinase activity (GO:0004672)protein localization to plasma membrane (GO:0072659)protein phosphorylation (GO:0006468)protein serine kinase activity (GO:0106310)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)regulation of calcium ion import (GO:0090279)regulation of monoatomic cation transmembrane transport (GO:1904062)regulation of monoatomic cation transmembrane transport (GO:1904062)regulation of monoatomic cation transmembrane transport (GO:1904062)regulation of monoatomic cation transmembrane transport (GO:1904062)renal sodium ion absorption (GO:0070294)transmembrane transporter binding (GO:0044325)transporter activator activity (GO:0141109)
Expression (TPM)
WNK3 — as a Regulated Gene

TFs regulating WNK3 0 TFs

Transcription factors with Perturb-seq knockdown data for WNK3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WNK3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to WNK3

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WNK3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chrX:54,182,249–54,183,769 174.6 kb Distal (>10kb) Multiome 570
chrX:54,357,469–54,359,170 68 bp At TSS Multiome 438
chrX:54,388,044–54,388,641 30.4 kb Distal (>10kb) Multiome 49
chrX:54,439,963–54,440,865 82.4 kb Distal (>10kb) Multiome 587
chrX:54,495,407–54,496,558 138.1 kb Distal (>10kb) Multiome 248
chrX:54,529,579–54,530,565 172.1 kb Distal (>10kb) Multiome 641
chrX:54,639,123–54,640,128 281.7 kb Distal (>10kb) Multiome 686

Genome Browser

Genomic view of the WNK3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chrX:54,172,249 – 54,650,128
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq