This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TOP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TOP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TOP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:40,949,667–40,950,138 | 79.0 kb | Distal (>10kb) Multiome | 26 | |
| chr20:40,990,562–40,991,029 | 38.1 kb | Distal (>10kb) Multiome | 486 | |
| chr20:41,023,627–41,023,825 | 5.0 kb | Proximal (<10kb) | 55 | |
| chr20:41,028,014–41,029,610 | 162 bp | At TSS Multiome | 977 | |
| chr20:41,135,300–41,135,808 | 106.6 kb | Distal (>10kb) Multiome | 451 | |
| chr20:41,136,345–41,138,443 | 108.4 kb | Distal (>10kb) Multiome | 1007 | |
| chr20:41,249,859–41,250,320 | 221.2 kb | Distal (>10kb) Multiome | 183 | |
| chr20:41,316,979–41,318,388 | 288.9 kb | Distal (>10kb) Multiome | 894 |
Genomic view of the TOP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.