Through alternative splicing, this gene encodes several distinct LEM domain containing protein isoforms. LEM domain proteins include inner nuclear membrane and intranuclear proteins, and are involved in a variety of cellular functions including gene expression, chromatin organization, and replication and cell cycle control. The encoded alpha isoform is broadly diffuse in the nucleus and contains a lamin binding domain, while the beta and gamma isoforms are localized to the nuclear membrane and contain an HDAC3 interaction domain. The distinct isoforms may compete with each other when acting to chaperone other proteins and regulate transcription. [provided by RefSeq, Aug 2019]
Transcription factors with Perturb-seq knockdown data for TMPO. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TMPO upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TMPO, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:98,291,819–98,292,796 | 223.5 kb | Distal (>10kb) Multiome | 111 | |
| chr12:98,344,774–98,345,967 | 170.2 kb | Distal (>10kb) Multiome | 190 | |
| chr12:98,397,273–98,398,042 | 118.0 kb | Distal (>10kb) Multiome | 455 | |
| chr12:98,456,620–98,457,422 | 58.5 kb | Distal (>10kb) Multiome | 319 | |
| chr12:98,490,969–98,491,482 | 24.4 kb | Distal (>10kb) Multiome HiCAR | 594 | |
| chr12:98,492,249–98,493,469 | 22.6 kb | Distal (>10kb) Multiome HiCAR | 235 | |
| chr12:98,502,866–98,504,417 | 11.8 kb | Distal (>10kb) Multiome HiCAR | 1073 | |
| chr12:98,508,872–98,509,583 | 6.0 kb | Proximal (<10kb) | 183 | |
| chr12:98,515,010–98,516,657 | 74 bp | At TSS Multiome | 999 | |
| chr12:98,520,559–98,520,845 | 5.0 kb | Proximal (<10kb) | 80 | |
| chr12:98,593,166–98,594,492 | 78.0 kb | Distal (>10kb) Multiome | 874 | |
| chr12:98,644,115–98,645,908 | 129.5 kb | Distal (>10kb) Multiome | 906 | |
| chr12:98,696,171–98,696,840 | 180.9 kb | Distal (>10kb) Multiome | 204 | |
| chr12:98,735,936–98,737,224 | 221.4 kb | Distal (>10kb) Multiome | 226 |
Genomic view of the TMPO locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.