This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain. [provided by RefSeq, Jul 2017]
Transcription factors with Perturb-seq knockdown data for TIAM1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TIAM1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TIAM1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr21:31,124,058–31,125,126 | 219.7 kb | Distal (>10kb) Multiome | 61 | |
| chr21:31,192,699–31,193,538 | 151.4 kb | Distal (>10kb) Multiome HiCAR | 73 | |
| chr21:31,343,630–31,344,641 | 212 bp | At TSS Multiome HiCAR | 366 | |
| chr21:31,348,236–31,348,494 | 3.9 kb | Proximal (<10kb) | 16 | |
| chr21:31,524,028–31,524,533 | 180.0 kb | Distal (>10kb) Multiome | 76 | |
| chr21:31,555,561–31,556,422 | 211.7 kb | Distal (>10kb) Multiome HiCAR | 195 | |
| chr21:31,557,394–31,560,223 | 215.0 kb | Distal (>10kb) Multiome HiCAR | 556 | |
| chr21:31,659,244–31,660,576 | 315.4 kb | Distal (>10kb) Multiome | 974 | |
| chr21:31,730,700–31,732,702 | 387.9 kb | Distal (>10kb) Multiome | 872 |
Genomic view of the TIAM1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.