The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TCF12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TCF12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TCF12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:56,464,770–56,465,573 | 453.5 kb | Distal (>10kb) Multiome HiCAR | 565 | |
| chr15:56,732,731–56,733,957 | 185.2 kb | Distal (>10kb) Multiome | 919 | |
| chr15:56,758,979–56,759,508 | 159.4 kb | Distal (>10kb) Multiome | 183 | |
| chr15:56,886,886–56,888,067 | 31.3 kb | Distal (>10kb) Multiome | 1086 | |
| chr15:56,917,829–56,920,157 | 112 bp | At TSS Multiome | 1031 | |
| chr15:57,198,642–57,199,172 | 280.2 kb | Distal (>10kb) Multiome | 193 | |
| chr15:57,219,294–57,219,476 | at TSS | At TSS | 84 | |
| chr15:57,255,638–57,256,396 | 337.4 kb | Distal (>10kb) Multiome | 74 | |
| chr15:57,259,456–57,260,042 | 341.1 kb | Distal (>10kb) Multiome | 261 | |
| chr15:57,306,141–57,307,417 | 388.1 kb | Distal (>10kb) Multiome | 512 | |
| chr15:57,321,974–57,322,475 | 403.7 kb | Distal (>10kb) Multiome | 391 | |
| chr15:57,323,712–57,324,672 | 405.7 kb | Distal (>10kb) Multiome | 392 | |
| chr15:57,376,103–57,377,146 | 457.8 kb | Distal (>10kb) Multiome | 482 | |
| chr15:57,382,704–57,383,499 | 464.6 kb | Distal (>10kb) Multiome | 134 | |
| chr15:57,385,937–57,386,391 | 467.5 kb | Distal (>10kb) Multiome | 106 | |
| chr15:57,411,681–57,412,959 | 493.8 kb | Distal (>10kb) Multiome | 165 | |
| chr15:57,477,799–57,478,810 | 559.7 kb | Distal (>10kb) Multiome | 603 | |
| chr15:57,495,641–57,496,411 | 577.4 kb | Distal (>10kb) Multiome | 71 | |
| chr15:57,501,832–57,502,700 | 583.6 kb | Distal (>10kb) Multiome | 59 |
Genomic view of the TCF12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.