TBX20
T-box transcription factor 20

This gene encodes a T-box family member. The T-box family members share a common DNA binding domain, termed the T-box, and they are transcription factors involved in the regulation of developmental processes. This gene is essential for heart development. Mutations in this gene are associated with diverse cardiac pathologies, including defects in septation, valvulogenesis and cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Member of: DE-4 DE-4.4 Developmental clusters: GC7
Biological processes 77 terms
DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)RNA polymerase II-specific DNA-binding transcription factor binding (GO:0061629)RNA polymerase II-specific DNA-binding transcription factor binding (GO:0061629)aortic valve development (GO:0003176)aortic valve morphogenesis (GO:0003180)atrial septum morphogenesis (GO:0060413)atrioventricular canal development (GO:0036302)atrioventricular canal development (GO:0036302)atrioventricular valve development (GO:0003171)atrioventricular valve development (GO:0003171)cardiac chamber formation (GO:0003207)cardiac muscle tissue morphogenesis (GO:0055008)cardiac muscle tissue morphogenesis (GO:0055008)cardiac right ventricle morphogenesis (GO:0003215)cardiac right ventricle morphogenesis (GO:0003215)cardiac septum development (GO:0003279)cardiac septum development (GO:0003279)cell fate specification (GO:0001708)chromatin (GO:0000785)chromatin (GO:0000785)cytoplasm (GO:0005737)cytoplasm (GO:0005737)dorsal/ventral pattern formation (GO:0009953)embryonic heart tube elongation (GO:0036306)embryonic heart tube morphogenesis (GO:0003143)embryonic heart tube morphogenesis (GO:0003143)endocardial cushion formation (GO:0003272)endocardial cushion formation (GO:0003272)endocardial cushion morphogenesis (GO:0003203)endocardial cushion morphogenesis (GO:0003203)endoderm formation (GO:0001706)foramen ovale closure (GO:0035922)heart looping (GO:0001947)heart looping (GO:0001947)heart valve morphogenesis (GO:0003179)lateral mesoderm formation (GO:0048370)mesenchymal cell development (GO:0014031)mesenchymal cell development (GO:0014031)negative regulation of DNA-templated transcription (GO:0045892)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)outflow tract morphogenesis (GO:0003151)outflow tract septum morphogenesis (GO:0003148)outflow tract septum morphogenesis (GO:0003148)pericardium morphogenesis (GO:0003344)pericardium morphogenesis (GO:0003344)positive regulation of BMP signaling pathway (GO:0030513)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of apoptotic process (GO:0043065)positive regulation of cardiac muscle cell proliferation (GO:0060045)positive regulation of cardiac muscle cell proliferation (GO:0060045)positive regulation of cell cycle process (GO:0090068)positive regulation of epithelial to mesenchymal transition (GO:0010718)positive regulation of transcription by RNA polymerase II (GO:0045944)pulmonary valve formation (GO:0003193)pulmonary valve formation (GO:0003193)pulmonary vein morphogenesis (GO:0060577)regulation of DNA-templated transcription (GO:0006355)regulation of transcription by RNA polymerase II (GO:0006357)sequence-specific double-stranded DNA binding (GO:1990837)tricuspid valve development (GO:0003175)tricuspid valve development (GO:0003175)visceral motor neuron differentiation (GO:0021524)visceral motor neuron differentiation (GO:0021524)
Expression (TPM)
TBX20 — as a Regulated Gene

TFs regulating TBX20 0 TFs

Transcription factors with Perturb-seq knockdown data for TBX20. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TBX20 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to TBX20

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TBX20, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr7:35,036,664–35,038,744 216.4 kb Distal (>10kb) Multiome 804
chr7:35,185,645–35,187,217 67.9 kb Distal (>10kb) Multiome 274
chr7:35,248,647–35,249,942 5.0 kb Proximal (<10kb) Multiome 358
chr7:35,253,386–35,255,107 47 bp At TSS Multiome 357
chr7:35,256,280–35,256,865 2.2 kb Proximal (<10kb) 76
chr7:35,257,289–35,258,565 3.2 kb Proximal (<10kb) 119
chr7:35,259,307–35,259,512 5.2 kb Proximal (<10kb) 16
chr7:35,261,015–35,262,351 7.8 kb Proximal (<10kb) Multiome 217
chr7:35,263,294–35,263,692 9.2 kb Proximal (<10kb) 24
chr7:35,301,809–35,303,376 48.3 kb Distal (>10kb) Multiome 85
chr7:35,331,849–35,332,680 78.1 kb Distal (>10kb) Multiome 175
chr7:35,390,142–35,390,653 136.3 kb Distal (>10kb) Multiome HiCAR 109
chr7:35,537,034–35,537,920 283.5 kb Distal (>10kb) Multiome HiCAR 411
chr7:35,624,084–35,625,028 370.5 kb Distal (>10kb) Multiome HiCAR 320

Genome Browser

Genomic view of the TBX20 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr7:35,026,664 – 35,635,028
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq