This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for SPAG5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPAG5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPAG5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:28,317,975–28,319,646 | 280.0 kb | Distal (>10kb) Multiome | 858 | |
| chr17:28,321,010–28,321,937 | 277.6 kb | Distal (>10kb) Multiome | 25 | |
| chr17:28,335,024–28,336,772 | 263.4 kb | Distal (>10kb) Multiome | 933 | |
| chr17:28,357,059–28,357,929 | 241.5 kb | Distal (>10kb) Multiome | 878 | |
| chr17:28,371,118–28,372,519 | 227.5 kb | Distal (>10kb) Multiome | 532 | |
| chr17:28,381,316–28,381,844 | 217.4 kb | Distal (>10kb) Multiome | 159 | |
| chr17:28,405,673–28,406,384 | 193.1 kb | Distal (>10kb) Multiome | 591 | |
| chr17:28,466,531–28,467,034 | 132.2 kb | Distal (>10kb) Multiome | 179 | |
| chr17:28,551,965–28,553,140 | 46.4 kb | Distal (>10kb) Multiome | 557 | |
| chr17:28,570,861–28,571,983 | 27.5 kb | Distal (>10kb) Multiome | 701 | |
| chr17:28,576,649–28,577,524 | 22.0 kb | Distal (>10kb) Multiome | 652 | |
| chr17:28,598,491–28,599,842 | 87 bp | At TSS Multiome | 810 | |
| chr17:28,621,936–28,622,562 | 23.3 kb | Distal (>10kb) Multiome | 55 | |
| chr17:28,644,541–28,645,596 | 46.2 kb | Distal (>10kb) Multiome | 795 | |
| chr17:28,661,635–28,662,527 | 63.1 kb | Distal (>10kb) Multiome | 809 | |
| chr17:28,710,720–28,712,813 | 112.4 kb | Distal (>10kb) Multiome | 892 | |
| chr17:28,717,030–28,718,784 | 118.9 kb | Distal (>10kb) Multiome | 492 | |
| chr17:28,718,918–28,720,313 | 120.8 kb | Distal (>10kb) Multiome | 927 | |
| chr17:28,724,652–28,729,474 | 128.0 kb | Distal (>10kb) Multiome | 1286 | |
| chr17:28,743,083–28,744,926 | 145.1 kb | Distal (>10kb) Multiome | 991 | |
| chr17:28,745,076–28,746,436 | 146.6 kb | Distal (>10kb) Multiome | 568 | |
| chr17:28,749,859–28,750,549 | 151.2 kb | Distal (>10kb) Multiome | 550 | |
| chr17:28,811,615–28,812,945 | 213.5 kb | Distal (>10kb) Multiome | 699 | |
| chr17:28,842,307–28,843,098 | 243.8 kb | Distal (>10kb) Multiome | 717 | |
| chr17:28,854,460–28,855,289 | 255.9 kb | Distal (>10kb) Multiome | 780 | |
| chr17:28,896,962–28,898,028 | 298.6 kb | Distal (>10kb) Multiome | 846 |
Genomic view of the SPAG5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.