The protein encoded by this gene is an SP family transcription factor that in mouse has been shown to be essential for proper limb development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
Transcription factors with Perturb-seq knockdown data for SP8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SP8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SP8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:20,653,525–20,654,195 | 133.0 kb | Distal (>10kb) Multiome | 116 | |
| chr7:20,700,321–20,701,121 | 86.1 kb | Distal (>10kb) Multiome | 96 | |
| chr7:20,775,903–20,777,464 | 10.3 kb | Distal (>10kb) Multiome | 266 | |
| chr7:20,777,629–20,779,374 | 8.6 kb | Proximal (<10kb) Multiome | 442 | |
| chr7:20,780,421–20,780,662 | 6.2 kb | Proximal (<10kb) | 18 | |
| chr7:20,783,229–20,783,425 | 3.5 kb | Proximal (<10kb) | 34 | |
| chr7:20,783,539–20,786,097 | 788 bp | At TSS | 393 | |
| chr7:20,786,445–20,787,645 | 139 bp | At TSS Multiome | 550 | |
| chr7:20,787,761–20,788,275 | 1.1 kb | Proximal (<10kb) Multiome | 79 | |
| chr7:20,790,922–20,791,759 | 4.0 kb | Proximal (<10kb) | 237 | |
| chr7:20,793,833–20,794,045 | 6.9 kb | Proximal (<10kb) | 61 | |
| chr7:20,795,975–20,796,174 | 9.1 kb | Proximal (<10kb) | 66 | |
| chr7:20,797,161–20,799,274 | 11.3 kb | Distal (>10kb) Multiome | 595 | |
| chr7:21,771,740–21,772,441 | 985.3 kb | Distal (>10kb) Multiome HiCAR | 259 |
Genomic view of the SP8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.