Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in this gene cause autosomal-dominant retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. [provided by RefSeq, Mar 2010]
Transcription factors with Perturb-seq knockdown data for SNRNP200. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SNRNP200 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SNRNP200, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:96,116,133–96,117,547 | 188.7 kb | Distal (>10kb) Multiome | 470 | |
| chr2:96,138,522–96,139,006 | 166.8 kb | Distal (>10kb) Multiome | 255 | |
| chr2:96,144,225–96,145,701 | 160.3 kb | Distal (>10kb) Multiome | 423 | |
| chr2:96,148,417–96,148,969 | 156.8 kb | Distal (>10kb) Multiome | 318 | |
| chr2:96,207,850–96,209,429 | 96.6 kb | Distal (>10kb) Multiome | 1022 | |
| chr2:96,265,014–96,266,655 | 39.4 kb | Distal (>10kb) Multiome | 992 | |
| chr2:96,305,302–96,305,947 | 62 bp | At TSS Multiome | 918 | |
| chr2:96,321,245–96,322,149 | 16.3 kb | Distal (>10kb) Multiome | 640 | |
| chr2:96,325,023–96,325,881 | 19.8 kb | Distal (>10kb) Multiome | 443 | |
| chr2:96,335,437–96,336,197 | 30.4 kb | Distal (>10kb) Multiome | 847 | |
| chr2:96,507,900–96,508,563 | 202.6 kb | Distal (>10kb) Multiome | 466 | |
| chr2:96,509,253–96,509,998 | 204.1 kb | Distal (>10kb) Multiome | 525 | |
| chr2:96,531,989–96,532,491 | 226.7 kb | Distal (>10kb) Multiome | 500 | |
| chr2:96,536,180–96,538,128 | 231.1 kb | Distal (>10kb) Multiome | 684 | |
| chr2:96,538,413–96,539,012 | 233.2 kb | Distal (>10kb) Multiome | 371 | |
| chr2:96,554,285–96,554,861 | 249.0 kb | Distal (>10kb) Multiome | 435 |
Genomic view of the SNRNP200 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.