Enables RNA binding activity and RNA polymerase II C-terminal domain phosphoserine binding activity. Involved in negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled and positive regulation of DNA-templated transcription, elongation. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for SCAF8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SCAF8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SCAF8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:154,509,167–154,511,379 | 222.7 kb | Distal (>10kb) Multiome | 984 | |
| chr6:154,542,675–154,543,191 | 190.4 kb | Distal (>10kb) Multiome | 171 | |
| chr6:154,645,535–154,646,800 | 87.1 kb | Distal (>10kb) Multiome | 96 | |
| chr6:154,652,848–154,653,591 | 80.2 kb | Distal (>10kb) Multiome | 127 | |
| chr6:154,732,598–154,734,903 | 721 bp | At TSS Multiome | 1110 | |
| chr6:154,742,961–154,743,189 | 9.5 kb | Proximal (<10kb) | 26 | |
| chr6:154,994,749–154,996,245 | 262.0 kb | Distal (>10kb) Multiome | 572 |
Genomic view of the SCAF8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.