SAE1

SUMO1 activating enzyme subunit 1 | AOS1, FLJ3091, Sua1

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Member of: DE-11 DE-11.4 Developmental clusters: GC1

Biological processes 26 terms

ATP-dependent protein binding (GO:0043008)SUMO activating enzyme activity (GO:0019948)SUMO activating enzyme activity (GO:0019948)SUMO activating enzyme complex (GO:0031510)SUMO activating enzyme complex (GO:0031510)SUMO activating enzyme complex (GO:0031510)cytoplasm (GO:0005737)cytosol (GO:0005829)enzyme activator activity (GO:0008047)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of protein localization to mitochondrion (GO:1903749)positive regulation of protein sumoylation (GO:0033235)protein binding (GO:0005515)protein heterodimerization activity (GO:0046982)protein sumoylation (GO:0016925)protein sumoylation (GO:0016925)protein sumoylation (GO:0016925)protein sumoylation (GO:0016925)protein ubiquitination (GO:0016567)small protein activating enzyme binding (GO:0044388)ubiquitin activating enzyme activity (GO:0004839)ubiquitin-like modifier activating enzyme activity (GO:0008641)

Expression (TPM)

TFs regulating SAE1 0 TFs

Transcription factors with Perturb-seq knockdown data for SAE1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SAE1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to SAE1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SAE1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr19:46,850,211–46,851,336	280.0 kb	Distal (>10kb) Multiome	946
chr19:46,855,920–46,856,711	274.6 kb	Distal (>10kb) Multiome	610
chr19:46,859,815–46,861,611	270.5 kb	Distal (>10kb) Multiome	1061
chr19:47,019,502–47,020,076	111.1 kb	Distal (>10kb) Multiome	188
chr19:47,035,599–47,036,293	94.8 kb	Distal (>10kb) Multiome	627
chr19:47,047,837–47,048,980	82.9 kb	Distal (>10kb) Multiome	760
chr19:47,058,561–47,059,308	71.9 kb	Distal (>10kb) Multiome	357
chr19:47,111,821–47,113,936	18.7 kb	Distal (>10kb) Multiome	1117
chr19:47,125,060–47,125,423	5.4 kb	Proximal (<10kb)	250
chr19:47,130,439–47,131,651	2 bp	At TSS Multiome	908
chr19:47,157,325–47,158,494	26.8 kb	Distal (>10kb) Multiome	271
chr19:47,196,586–47,197,452	66.0 kb	Distal (>10kb) Multiome	94
chr19:47,225,386–47,231,990	97.0 kb	Distal (>10kb) Multiome HiCAR	1389
chr19:47,240,827–47,241,572	110.4 kb	Distal (>10kb) Multiome HiCAR	616
chr19:47,242,713–47,243,645	112.3 kb	Distal (>10kb) Multiome HiCAR	568
chr19:47,243,835–47,244,896	113.5 kb	Distal (>10kb) Multiome HiCAR	1001
chr19:47,256,041–47,258,057	126.3 kb	Distal (>10kb) Multiome HiCAR	907
chr19:47,272,778–47,275,890	142.6 kb	Distal (>10kb) Multiome	931
chr19:47,348,695–47,349,758	218.4 kb	Distal (>10kb) Multiome	766
chr19:47,406,825–47,407,408	276.5 kb	Distal (>10kb) Multiome	333
chr19:47,427,132–47,428,809	296.4 kb	Distal (>10kb) Multiome	506
chr19:47,429,865–47,430,531	299.5 kb	Distal (>10kb) Multiome	438

Genome Browser

Genomic view of the SAE1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.