Enables MDM2/MDM4 family protein binding activity; p53 binding activity; and ubiquitin protein ligase activity. Involved in several processes, including DNA metabolic process; regulation of cell cycle phase transition; and response to ionizing radiation. Located in nucleoplasm and site of double-strand break. Implicated in Fanconi anemia complementation group W. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for RFWD3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFWD3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFWD3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:74,367,658–74,368,475 | 298.7 kb | Distal (>10kb) Multiome | 473 | |
| chr16:74,495,325–74,495,916 | 171.3 kb | Distal (>10kb) Multiome | 90 | |
| chr16:74,606,340–74,607,641 | 59.8 kb | Distal (>10kb) Multiome | 1020 | |
| chr16:74,666,411–74,667,145 | 70 bp | At TSS Multiome | 858 | |
| chr16:74,667,439–74,667,608 | 562 bp | At TSS | 303 | |
| chr16:74,700,393–74,701,030 | 33.8 kb | Distal (>10kb) Multiome | 785 | |
| chr16:74,774,151–74,775,199 | 107.9 kb | Distal (>10kb) Multiome | 669 | |
| chr16:74,812,990–74,813,481 | 146.4 kb | Distal (>10kb) Multiome | 680 | |
| chr16:74,866,003–74,866,478 | 199.3 kb | Distal (>10kb) Multiome | 95 |
Genomic view of the RFWD3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.