Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for RAPGEF2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAPGEF2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAPGEF2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:158,902,258–158,903,267 | 200.3 kb | Distal (>10kb) Multiome | 354 | |
| chr4:159,102,093–159,105,044 | 97 bp | At TSS Multiome | 843 | |
| chr4:159,105,147–159,105,452 | 2.1 kb | Proximal (<10kb) | 98 | |
| chr4:159,105,638–159,106,232 | 2.6 kb | Proximal (<10kb) | 211 | |
| chr4:159,382,270–159,384,010 | 280.6 kb | Distal (>10kb) Multiome | 320 |
Genomic view of the RAPGEF2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.