The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PRRX1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRRX1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRRX1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:170,660,158–170,660,691 | 2.4 kb | Proximal (<10kb) | 60 | |
| chr1:170,660,944–170,662,628 | 504 bp | At TSS | 217 | |
| chr1:170,662,985–170,663,591 | at TSS | At TSS | 84 | |
| chr1:170,663,964–170,665,497 | 831 bp | At TSS | 357 | |
| chr1:170,665,688–170,666,019 | 2.6 kb | Proximal (<10kb) | 48 | |
| chr1:170,666,646–170,667,067 | 3.5 kb | Proximal (<10kb) | 86 | |
| chr1:170,667,162–170,667,706 | 4.0 kb | Proximal (<10kb) | 75 | |
| chr1:170,668,159–170,669,041 | 5.0 kb | Proximal (<10kb) | 182 | |
| chr1:170,671,189–170,671,827 | 8.1 kb | Proximal (<10kb) | 133 |
Genomic view of the PRRX1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.