Transcription factors with Perturb-seq knockdown data for PRDM8-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRDM8-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRDM8-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:80,179,621–80,180,177 | 10.0 kb | Proximal (<10kb) | 167 | |
| chr4:80,182,435–80,182,804 | 7.4 kb | Proximal (<10kb) | 50 | |
| chr4:80,183,397–80,184,999 | 5.2 kb | Proximal (<10kb) | 610 | |
| chr4:80,185,441–80,185,658 | 4.5 kb | Proximal (<10kb) | 59 | |
| chr4:80,188,776–80,189,299 | 869 bp | At TSS | 44 | |
| chr4:80,189,905–80,190,753 | at TSS | At TSS | 185 | |
| chr4:80,196,357–80,196,578 | 6.2 kb | Proximal (<10kb) | 113 | |
| chr4:80,196,927–80,198,313 | 6.8 kb | Proximal (<10kb) | 272 |
Genomic view of the PRDM8-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.