The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
Transcription factors with Perturb-seq knockdown data for PPP1R16B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPP1R16B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPP1R16B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:38,607,297–38,607,885 | 198.2 kb | Distal (>10kb) Multiome | 125 | |
| chr20:38,612,897–38,613,663 | 192.5 kb | Distal (>10kb) Multiome | 78 | |
| chr20:38,646,005–38,646,460 | 159.4 kb | Distal (>10kb) Multiome | 567 | |
| chr20:38,673,746–38,675,067 | 131.2 kb | Distal (>10kb) Multiome | 446 | |
| chr20:38,725,938–38,726,412 | 79.7 kb | Distal (>10kb) Multiome | 218 | |
| chr20:38,727,220–38,727,813 | 78.3 kb | Distal (>10kb) Multiome | 231 | |
| chr20:38,730,955–38,731,938 | 74.3 kb | Distal (>10kb) Multiome | 646 | |
| chr20:38,747,701–38,748,998 | 57.5 kb | Distal (>10kb) Multiome | 909 | |
| chr20:38,804,662–38,806,744 | 839 bp | At TSS Multiome | 532 | |
| chr20:38,825,817–38,826,402 | 20.5 kb | Distal (>10kb) Multiome | 502 | |
| chr20:38,893,515–38,894,286 | 88.3 kb | Distal (>10kb) Multiome | 473 | |
| chr20:38,925,940–38,927,103 | 120.6 kb | Distal (>10kb) Multiome | 989 | |
| chr20:38,961,917–38,962,858 | 156.6 kb | Distal (>10kb) Multiome | 1011 | |
| chr20:39,049,709–39,050,723 | 244.5 kb | Distal (>10kb) Multiome | 898 |
Genomic view of the PPP1R16B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.