POLR2B

RNA polymerase II subunit B | RPB2

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Member of: DE-5 Developmental clusters: GC4

Biological processes 27 terms

5'-3' RNA polymerase activity (GO:0034062)DNA binding (GO:0003677)DNA binding (GO:0003677)DNA-directed RNA polymerase activity (GO:0003899)DNA-directed RNA polymerase activity (GO:0003899)DNA-directed RNA polymerase activity (GO:0003899)DNA-templated transcription (GO:0006351)DNA-templated transcription elongation (GO:0006354)RNA binding (GO:0003723)RNA polymerase II, core complex (GO:0005665)RNA polymerase II, core complex (GO:0005665)RNA-directed RNA polymerase activity (GO:0003968)RNA-directed RNA polymerase activity (GO:0003968)RNA-templated transcription (GO:0001172)chromatin binding (GO:0003682)chromosome, telomeric region (GO:0000781)membrane (GO:0016020)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)protein binding (GO:0005515)ribonucleoside binding (GO:0032549)transcription by RNA polymerase II (GO:0006366)transcription elongation by RNA polymerase II (GO:0006368)transcription initiation at RNA polymerase II promoter (GO:0006367)transcription initiation at RNA polymerase II promoter (GO:0006367)

Expression (TPM)

TFs regulating POLR2B 0 TFs

Transcription factors with Perturb-seq knockdown data for POLR2B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POLR2B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to POLR2B

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POLR2B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr4:56,787,104–56,787,870	190.3 kb	Distal (>10kb) Multiome	101
chr4:56,821,230–56,822,148	156.0 kb	Distal (>10kb) Multiome	302
chr4:56,844,583–56,845,457	132.7 kb	Distal (>10kb) Multiome	178
chr4:56,906,574–56,909,582	69.9 kb	Distal (>10kb) Multiome	887
chr4:56,976,336–56,979,242	107 bp	At TSS Multiome	954
chr4:56,979,552–56,979,914	1.8 kb	Proximal (<10kb)	75
chr4:57,037,888–57,038,581	60.5 kb	Distal (>10kb) Multiome	261
chr4:57,109,714–57,111,367	132.6 kb	Distal (>10kb) Multiome	438
chr4:57,163,440–57,164,715	186.2 kb	Distal (>10kb) Multiome	642
chr4:57,190,721–57,192,448	214.1 kb	Distal (>10kb) Multiome	348

Genome Browser

Genomic view of the POLR2B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.