PLCG1
phospholipase C gamma 1 | NCKAP3, PLC-II, PLC148, PLCgamma1, PLC1

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Member of: DE-2 DE-2.3 Developmental clusters: GC6
Biological processes 58 terms
C-type glycerophospholipase activity (GO:0004629)C-type glycerophospholipase activity (GO:0004629)C-type glycerophospholipase activity (GO:0004629)COP9 signalosome (GO:0008180)Fc-epsilon receptor signaling pathway (GO:0038095)Fc-epsilon receptor signaling pathway (GO:0038095)T cell receptor signaling pathway (GO:0050852)antigen receptor-mediated signaling pathway (GO:0050851)calcium ion binding (GO:0005509)calcium-mediated signaling (GO:0019722)calcium-mediated signaling (GO:0019722)cell migration (GO:0016477)cell projection (GO:0042995)cellular response to epidermal growth factor stimulus (GO:0071364)cellular response to epidermal growth factor stimulus (GO:0071364)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)epidermal growth factor receptor signaling pathway (GO:0007173)epidermal growth factor receptor signaling pathway (GO:0007173)guanyl-nucleotide exchange factor activity (GO:0005085)intracellular signal transduction (GO:0035556)lamellipodium (GO:0030027)lamellipodium (GO:0030027)lipid metabolic process (GO:0006629)lysophospholipase C activity (GO:0140324)negative regulation of inflammatory response to antigenic stimulus (GO:0002862)neurotrophin TRKA receptor binding (GO:0005168)phosphatidylinositol metabolic process (GO:0046488)phosphatidylinositol metabolic process (GO:0046488)phosphatidylinositol phospholipase C activity (GO:0120548)phosphatidylinositol-4,5-bisphosphate phospholipase C activity (GO:0004435)phosphatidylinositol-4,5-bisphosphate phospholipase C activity (GO:0004435)phosphatidylinositol-4,5-bisphosphate phospholipase C activity (GO:0004435)phosphatidylinositol-4,5-bisphosphate phospholipase C activity (GO:0004435)phosphatidylinositol-mediated signaling (GO:0048015)phospholipid catabolic process (GO:0009395)phosphoric diester hydrolase activity (GO:0008081)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of angiogenesis (GO:0045766)positive regulation of blood vessel endothelial cell migration (GO:0043536)positive regulation of endothelial cell apoptotic process (GO:2000353)positive regulation of epithelial cell migration (GO:0010634)positive regulation of epithelial cell migration (GO:0010634)positive regulation of release of sequestered calcium ion into cytosol (GO:0051281)positive regulation of vascular endothelial cell proliferation (GO:1905564)protein binding (GO:0005515)protein kinase binding (GO:0019901)release of sequestered calcium ion into cytosol (GO:0051209)ruffle (GO:0001726)ruffle (GO:0001726)ruffle membrane (GO:0032587)ruffle membrane (GO:0032587)ruffle membrane (GO:0032587)signal transduction (GO:0007165)
Expression (TPM)
PLCG1 — as a Regulated Gene

TFs regulating PLCG1 0 TFs

Transcription factors with Perturb-seq knockdown data for PLCG1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PLCG1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to PLCG1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PLCG1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr20:40,949,667–40,950,138 187.7 kb Distal (>10kb) Multiome 26
chr20:40,990,562–40,991,029 146.8 kb Distal (>10kb) Multiome 486
chr20:41,028,014–41,029,610 108.9 kb Distal (>10kb) Multiome 977
chr20:41,135,300–41,135,808 2.1 kb Proximal (<10kb) Multiome 451
chr20:41,136,345–41,138,443 296 bp At TSS Multiome 1007
chr20:41,249,859–41,250,320 112.5 kb Distal (>10kb) Multiome 183
chr20:41,316,979–41,318,388 180.2 kb Distal (>10kb) Multiome 894
chr20:41,339,588–41,341,431 203.2 kb Distal (>10kb) Multiome 904
chr20:41,365,890–41,367,375 229.3 kb Distal (>10kb) Multiome 273

Genome Browser

Genomic view of the PLCG1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr20:40,939,667 – 41,377,375
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq