PIWIL2

piwi like RNA-mediated gene silencing 2 | CT80, FLJ10351, HILI, Mili

PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]

Developmental clusters: GC4

Biological processes 50 terms

P granule (GO:0043186)P granule (GO:0043186)P granule (GO:0043186)PET complex (GO:1990923)PET complex (GO:1990923)RNA binding (GO:0003723)RNA endonuclease activity (GO:0004521)RNA endonuclease activity (GO:0004521)RNA endonuclease activity (GO:0004521)chromatoid body (GO:0033391)chromatoid body (GO:0033391)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)dense body (GO:0097433)germ-line stem cell population maintenance (GO:0030718)germ-line stem cell population maintenance (GO:0030718)mRNA binding (GO:0003729)negative regulation of circadian rhythm (GO:0042754)negative regulation of circadian rhythm (GO:0042754)nucleic acid binding (GO:0003676)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)oogenesis (GO:0048477)oogenesis (GO:0048477)perinucleolar chromocenter (GO:0010370)pi-body (GO:0071546)pi-body (GO:0071546)piRNA binding (GO:0034584)piRNA binding (GO:0034584)piRNA binding (GO:0034584)piRNA binding (GO:0034584)piRNA processing (GO:0034587)piRNA processing (GO:0034587)piRNA-mediated gene silencing by mRNA destabilization (GO:0140991)positive regulation of cytoplasmic translation (GO:2000767)positive regulation of cytoplasmic translation (GO:2000767)protein binding (GO:0005515)regulatory ncRNA-mediated gene silencing (GO:0031047)regulatory ncRNA-mediated gene silencing (GO:0031047)regulatory ncRNA-mediated gene silencing (GO:0031047)secondary piRNA processing (GO:0140965)spermatogenesis (GO:0007283)spermatogenesis (GO:0007283)spermatogenesis (GO:0007283)transposable element silencing by heterochromatin formation (GO:0141005)transposable element silencing by mRNA destabilization (GO:0141008)transposable element silencing by piRNA-mediated DNA methylation (GO:0141196)transposable element silencing by piRNA-mediated DNA methylation (GO:0141196)

Expression (TPM)

TFs regulating PIWIL2 0 TFs

Transcription factors with Perturb-seq knockdown data for PIWIL2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PIWIL2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to PIWIL2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PIWIL2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr8:22,009,978–22,010,924	264.9 kb	Distal (>10kb) Multiome	885
chr8:22,024,029–22,025,514	250.1 kb	Distal (>10kb) Multiome	508
chr8:22,036,665–22,037,480	238.3 kb	Distal (>10kb) Multiome	457
chr8:22,039,647–22,043,111	235.3 kb	Distal (>10kb) Multiome	407
chr8:22,047,856–22,051,849	226.1 kb	Distal (>10kb) Multiome	566
chr8:22,052,727–22,057,283	221.3 kb	Distal (>10kb) Multiome	541
chr8:22,066,266–22,067,293	208.4 kb	Distal (>10kb) Multiome	612
chr8:22,069,764–22,070,311	205.2 kb	Distal (>10kb) Multiome	85
chr8:22,089,006–22,089,746	186.1 kb	Distal (>10kb) Multiome	562
chr8:22,095,301–22,095,802	179.7 kb	Distal (>10kb) Multiome	199
chr8:22,108,625–22,110,515	165.8 kb	Distal (>10kb) Multiome	907
chr8:22,129,779–22,131,071	144.8 kb	Distal (>10kb) Multiome	440
chr8:22,137,231–22,137,850	137.8 kb	Distal (>10kb) Multiome	531
chr8:22,140,995–22,142,545	133.4 kb	Distal (>10kb) Multiome	753
chr8:22,156,517–22,157,091	118.5 kb	Distal (>10kb) Multiome	215
chr8:22,164,354–22,165,830	110.3 kb	Distal (>10kb) Multiome	672
chr8:22,176,636–22,177,193	98.3 kb	Distal (>10kb) Multiome	91
chr8:22,188,487–22,189,031	86.5 kb	Distal (>10kb) Multiome	334
chr8:22,244,388–22,245,751	30.2 kb	Distal (>10kb) Multiome	1013
chr8:22,275,120–22,276,011	50 bp	At TSS Multiome	195
chr8:22,365,087–22,366,071	90.3 kb	Distal (>10kb) Multiome	444
chr8:22,366,837–22,368,383	92.0 kb	Distal (>10kb) Multiome	859
chr8:22,440,466–22,441,769	165.8 kb	Distal (>10kb) Multiome	784
chr8:22,548,856–22,550,046	274.2 kb	Distal (>10kb) Multiome	539
chr8:22,551,208–22,552,929	276.3 kb	Distal (>10kb) Multiome	406
chr8:22,561,173–22,562,479	286.5 kb	Distal (>10kb) Multiome	678
chr8:22,565,061–22,566,429	290.4 kb	Distal (>10kb) Multiome	404
chr8:22,574,221–22,574,708	299.2 kb	Distal (>10kb) Multiome	527

Genome Browser

Genomic view of the PIWIL2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.