The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
Transcription factors with Perturb-seq knockdown data for PFKFB3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PFKFB3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PFKFB3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:5,866,673–5,867,954 | 277.6 kb | Distal (>10kb) Multiome | 153 | |
| chr10:5,888,755–5,891,014 | 254.9 kb | Distal (>10kb) Multiome | 918 | |
| chr10:5,976,561–5,978,337 | 167.0 kb | Distal (>10kb) Multiome | 723 | |
| chr10:6,086,345–6,087,026 | 58.4 kb | Distal (>10kb) Multiome | 467 | |
| chr10:6,088,532–6,090,276 | 56.0 kb | Distal (>10kb) Multiome | 847 | |
| chr10:6,108,563–6,109,206 | 36.1 kb | Distal (>10kb) Multiome HiCAR | 207 | |
| chr10:6,144,513–6,146,019 | 23 bp | At TSS Multiome | 592 | |
| chr10:6,151,133–6,151,901 | 6.6 kb | Proximal (<10kb) Multiome | 300 | |
| chr10:6,162,653–6,162,919 | 912 bp | At TSS | 95 | |
| chr10:6,163,113–6,163,281 | 550 bp | At TSS | 80 | |
| chr10:6,163,382–6,164,228 | 18.8 kb | Distal (>10kb) Multiome | 706 | |
| chr10:6,184,834–6,185,745 | 40.4 kb | Distal (>10kb) Multiome | 123 | |
| chr10:6,200,717–6,205,207 | 56.6 kb | Distal (>10kb) Multiome | 1010 |
Genomic view of the PFKFB3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.