This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NUF2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NUF2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NUF2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:163,069,170–163,069,674 | 252.6 kb | Distal (>10kb) Multiome HiCAR | 172 | |
| chr1:163,195,927–163,196,435 | 125.8 kb | Distal (>10kb) Multiome | 275 | |
| chr1:163,202,883–163,203,536 | 118.8 kb | Distal (>10kb) Multiome | 178 | |
| chr1:163,312,429–163,312,953 | 9.0 kb | Proximal (<10kb) | 17 | |
| chr1:163,321,363–163,322,459 | 32 bp | At TSS Multiome | 1184 | |
| chr1:163,399,976–163,400,589 | 78.3 kb | Distal (>10kb) Multiome | 146 | |
| chr1:163,561,453–163,562,798 | 240.1 kb | Distal (>10kb) Multiome | 254 | |
| chr1:163,590,875–163,591,911 | 269.5 kb | Distal (>10kb) Multiome | 191 | |
| chr1:163,595,781–163,597,181 | 274.4 kb | Distal (>10kb) Multiome | 255 | |
| chr1:164,774,689–164,775,602 | 1453.2 kb | Distal (>10kb) Multiome HiCAR | 367 |
Genomic view of the NUF2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.