This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis. [provided by RefSeq, Aug 2016]
Transcription factors with Perturb-seq knockdown data for NODAL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NODAL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NODAL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:70,145,328–70,147,235 | 296.0 kb | Distal (>10kb) Multiome | 1006 | |
| chr10:70,169,902–70,171,034 | 271.1 kb | Distal (>10kb) Multiome | 777 | |
| chr10:70,232,204–70,234,260 | 208.1 kb | Distal (>10kb) Multiome | 949 | |
| chr10:70,381,835–70,383,091 | 59.0 kb | Distal (>10kb) Multiome | 693 | |
| chr10:70,403,687–70,404,709 | 37.6 kb | Distal (>10kb) Multiome | 788 | |
| chr10:70,437,649–70,438,147 | 3.7 kb | Proximal (<10kb) Multiome | 93 | |
| chr10:70,439,787–70,443,007 | 970 bp | At TSS Multiome | 501 | |
| chr10:70,445,771–70,446,449 | 4.1 kb | Proximal (<10kb) | 87 | |
| chr10:70,450,965–70,451,354 | 9.3 kb | Proximal (<10kb) | 65 | |
| chr10:70,455,031–70,455,839 | 13.8 kb | Distal (>10kb) Multiome | 196 | |
| chr10:70,458,264–70,459,984 | 16.9 kb | Distal (>10kb) Multiome | 310 | |
| chr10:70,478,010–70,479,323 | 37.0 kb | Distal (>10kb) Multiome | 492 | |
| chr10:70,672,118–70,672,962 | 230.9 kb | Distal (>10kb) Multiome | 465 |
Genomic view of the NODAL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.