This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016]
Transcription factors with Perturb-seq knockdown data for NNT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NNT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NNT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:43,312,549–43,314,340 | 289.1 kb | Distal (>10kb) Multiome | 954 | |
| chr5:43,348,196–43,348,656 | 254.3 kb | Distal (>10kb) Multiome | 141 | |
| chr5:43,396,804–43,397,322 | 205.6 kb | Distal (>10kb) Multiome | 340 | |
| chr5:43,416,225–43,416,784 | 186.2 kb | Distal (>10kb) Multiome | 263 | |
| chr5:43,483,333–43,484,741 | 118.8 kb | Distal (>10kb) Multiome | 1033 | |
| chr5:43,514,568–43,515,502 | 87.6 kb | Distal (>10kb) Multiome | 817 | |
| chr5:43,555,878–43,557,718 | 45.8 kb | Distal (>10kb) Multiome | 936 | |
| chr5:43,602,369–43,604,281 | 412 bp | At TSS Multiome | 912 | |
| chr5:43,607,002–43,607,875 | 4.3 kb | Proximal (<10kb) | 24 | |
| chr5:44,506,221–44,506,892 | 904.0 kb | Distal (>10kb) Multiome HiCAR | 207 | |
| chr5:44,638,090–44,639,138 | 1035.9 kb | Distal (>10kb) Multiome HiCAR | 164 |
Genomic view of the NNT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.