This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
Transcription factors with Perturb-seq knockdown data for NET1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NET1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NET1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:4,825,541–4,827,059 | 620.2 kb | Distal (>10kb) Multiome HiCAR | 564 | |
| chr10:5,293,780–5,294,676 | 152.3 kb | Distal (>10kb) Multiome | 268 | |
| chr10:5,412,181–5,413,375 | 34.0 kb | Distal (>10kb) Multiome | 650 | |
| chr10:5,416,686–5,417,229 | 4.1 kb | Proximal (<10kb) | 74 | |
| chr10:5,446,256–5,446,879 | 28 bp | At TSS Multiome | 855 | |
| chr10:5,447,641–5,447,987 | 1.0 kb | Proximal (<10kb) | 199 | |
| chr10:5,466,039–5,467,339 | 20.2 kb | Distal (>10kb) Multiome | 493 | |
| chr10:5,472,263–5,472,901 | 25.9 kb | Distal (>10kb) Multiome | 181 | |
| chr10:5,623,013–5,623,598 | 176.7 kb | Distal (>10kb) Multiome HiCAR | 611 | |
| chr10:5,665,943–5,666,966 | 220.0 kb | Distal (>10kb) Multiome | 720 | |
| chr10:5,684,325–5,685,785 | 238.1 kb | Distal (>10kb) Multiome | 1140 | |
| chr10:5,692,454–5,693,306 | 246.3 kb | Distal (>10kb) Multiome | 757 | |
| chr10:5,697,995–5,698,400 | 251.5 kb | Distal (>10kb) Multiome | 69 |
Genomic view of the NET1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.