This gene likely encodes a member of the adaptin-ear-binding coat-associated protein family. Studies of a similar protein in rat suggest a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]
Transcription factors with Perturb-seq knockdown data for NECAP2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NECAP2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NECAP2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:16,145,972–16,147,046 | 294.3 kb | Distal (>10kb) Multiome | 689 | |
| chr1:16,154,818–16,156,461 | 284.7 kb | Distal (>10kb) Multiome | 742 | |
| chr1:16,167,178–16,168,479 | 272.9 kb | Distal (>10kb) Multiome | 501 | |
| chr1:16,206,128–16,208,181 | 234.0 kb | Distal (>10kb) Multiome | 760 | |
| chr1:16,212,441–16,212,918 | 228.0 kb | Distal (>10kb) Multiome | 183 | |
| chr1:16,215,805–16,218,073 | 223.2 kb | Distal (>10kb) Multiome | 490 | |
| chr1:16,226,476–16,227,566 | 213.7 kb | Distal (>10kb) Multiome | 208 | |
| chr1:16,236,291–16,237,528 | 203.6 kb | Distal (>10kb) Multiome | 717 | |
| chr1:16,351,966–16,353,171 | 88.2 kb | Distal (>10kb) Multiome | 894 | |
| chr1:16,366,922–16,368,057 | 73.5 kb | Distal (>10kb) Multiome | 957 | |
| chr1:16,440,240–16,440,992 | 122 bp | At TSS Multiome | 936 | |
| chr1:16,498,731–16,499,831 | 58.5 kb | Distal (>10kb) Multiome | 710 | |
| chr1:16,513,524–16,514,536 | 73.3 kb | Distal (>10kb) Multiome | 716 | |
| chr1:16,534,972–16,535,673 | 94.6 kb | Distal (>10kb) Multiome | 146 | |
| chr1:16,612,236–16,614,194 | 172.9 kb | Distal (>10kb) Multiome | 1105 | |
| chr1:16,620,707–16,621,639 | 180.4 kb | Distal (>10kb) Multiome | 685 | |
| chr1:16,639,254–16,639,764 | 198.8 kb | Distal (>10kb) Multiome | 46 | |
| chr1:16,644,020–16,645,162 | 204.0 kb | Distal (>10kb) Multiome | 979 | |
| chr1:16,727,237–16,727,897 | 286.6 kb | Distal (>10kb) Multiome | 430 |
Genomic view of the NECAP2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.