The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]
Transcription factors with Perturb-seq knockdown data for NCK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NCK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NCK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:136,751,181–136,753,220 | 109.7 kb | Distal (>10kb) Multiome | 1046 | |
| chr3:136,818,569–136,819,674 | 43.2 kb | Distal (>10kb) Multiome | 656 | |
| chr3:136,819,787–136,820,421 | 42.0 kb | Distal (>10kb) Multiome | 524 | |
| chr3:136,860,443–136,860,889 | 1.3 kb | Proximal (<10kb) | 111 | |
| chr3:136,861,045–136,862,941 | 53 bp | At TSS Multiome | 966 | |
| chr3:137,032,463–137,033,394 | 170.5 kb | Distal (>10kb) Multiome | 351 |
Genomic view of the NCK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.