Transcription factors with Perturb-seq knockdown data for MYLK-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MYLK-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MYLK-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:123,314,223–123,315,571 | 270.3 kb | Distal (>10kb) Multiome | 264 | |
| chr3:123,447,574–123,450,476 | 137.2 kb | Distal (>10kb) Multiome | 427 | |
| chr3:123,571,319–123,571,775 | 13.7 kb | Distal (>10kb) Multiome | 101 | |
| chr3:123,584,314–123,585,841 | 124 bp | At TSS Multiome | 988 | |
| chr3:123,586,059–123,586,522 | 760 bp | At TSS | 281 | |
| chr3:123,588,179–123,588,401 | 2.9 kb | Proximal (<10kb) | 11 | |
| chr3:123,600,718–123,601,402 | 15.8 kb | Distal (>10kb) Multiome | 74 | |
| chr3:123,636,347–123,636,942 | 51.4 kb | Distal (>10kb) Multiome | 211 | |
| chr3:123,883,672–123,884,952 | 299.2 kb | Distal (>10kb) Multiome | 407 |
Genomic view of the MYLK-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.