MXD4

MAX dimerization protein 4 | MAD4, MST149, MSTP149, bHLHc12

This gene is a member of the MAD gene family . The MAD genes encode basic helix-loop-helix-leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues. [provided by RefSeq, Jul 2008]

Member of: DE-4 DE-4.8 Developmental clusters: GC6

Biological processes 14 terms

DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)chromatin (GO:0000785)cytosol (GO:0005829)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)protein binding (GO:0005515)protein dimerization activity (GO:0046983)regulation of transcription by RNA polymerase II (GO:0006357)

Expression (TPM)

TFs regulating MXD4 0 TFs

Transcription factors with Perturb-seq knockdown data for MXD4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MXD4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to MXD4

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MXD4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr4:2,008,507–2,010,125	252.9 kb	Distal (>10kb) Multiome	1040
chr4:2,041,030–2,042,663	220.1 kb	Distal (>10kb) Multiome	554
chr4:2,060,701–2,061,398	201.0 kb	Distal (>10kb) Multiome	197
chr4:2,241,509–2,242,666	19.9 kb	Distal (>10kb) Multiome	783
chr4:2,261,141–2,263,069	236 bp	At TSS Multiome	806
chr4:2,271,300–2,271,520	9.2 kb	Proximal (<10kb)	32
chr4:2,401,939–2,402,479	140.2 kb	Distal (>10kb) Multiome HiCAR	317
chr4:2,413,008–2,413,630	151.3 kb	Distal (>10kb) Multiome HiCAR	564
chr4:2,417,912–2,419,213	156.7 kb	Distal (>10kb) Multiome HiCAR	628
chr4:2,462,245–2,463,183	200.6 kb	Distal (>10kb) Multiome	643
chr4:2,468,399–2,469,652	206.9 kb	Distal (>10kb) Multiome	858
chr4:2,535,307–2,537,785	274.3 kb	Distal (>10kb) Multiome	860

Genome Browser

Genomic view of the MXD4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.