This gene encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. This protein has an affinity for Glc2Man9GlcNAc2 (G2M9) N-glycans and is involved in regulating glycosylation in the endoplasmic reticulum. This protein has also been shown to interact with ribophorin I and may be involved in the directing the degradation of misfolded proteins. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Transcription factors with Perturb-seq knockdown data for MLEC. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MLEC upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MLEC, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:120,388,762–120,389,560 | 297.9 kb | Distal (>10kb) Multiome | 178 | |
| chr12:120,394,470–120,395,052 | 292.4 kb | Distal (>10kb) Multiome | 283 | |
| chr12:120,413,842–120,414,516 | 272.9 kb | Distal (>10kb) Multiome | 289 | |
| chr12:120,437,436–120,438,568 | 249.1 kb | Distal (>10kb) Multiome | 725 | |
| chr12:120,445,927–120,446,970 | 240.7 kb | Distal (>10kb) Multiome | 982 | |
| chr12:120,468,864–120,470,390 | 217.4 kb | Distal (>10kb) Multiome | 942 | |
| chr12:120,495,362–120,496,939 | 191.1 kb | Distal (>10kb) Multiome | 794 | |
| chr12:120,528,856–120,529,468 | 158.0 kb | Distal (>10kb) Multiome | 849 | |
| chr12:120,534,230–120,535,077 | 152.6 kb | Distal (>10kb) Multiome | 730 | |
| chr12:120,580,919–120,581,830 | 105.7 kb | Distal (>10kb) Multiome HiCAR | 736 | |
| chr12:120,584,329–120,585,045 | 102.4 kb | Distal (>10kb) Multiome HiCAR | 460 | |
| chr12:120,640,005–120,641,704 | 46.7 kb | Distal (>10kb) Multiome HiCAR | 562 | |
| chr12:120,648,914–120,649,665 | 37.8 kb | Distal (>10kb) Multiome HiCAR | 405 | |
| chr12:120,668,575–120,669,135 | 18.3 kb | Distal (>10kb) Multiome | 98 | |
| chr12:120,686,185–120,687,741 | 368 bp | At TSS Multiome | 1030 | |
| chr12:120,710,025–120,711,277 | 23.2 kb | Distal (>10kb) Multiome HiCAR | 573 | |
| chr12:120,725,271–120,726,309 | 38.7 kb | Distal (>10kb) Multiome | 663 | |
| chr12:120,822,842–120,823,717 | 136.0 kb | Distal (>10kb) Multiome | 117 | |
| chr12:120,903,538–120,904,786 | 217.3 kb | Distal (>10kb) Multiome | 749 | |
| chr12:121,016,022–121,016,705 | 329.3 kb | Distal (>10kb) Multiome HiCAR | 674 |
Genomic view of the MLEC locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.