This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MDFI. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MDFI upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MDFI, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:41,373,601–41,374,643 | 262.6 kb | Distal (>10kb) Multiome | 200 | |
| chr6:41,406,787–41,407,231 | 229.8 kb | Distal (>10kb) Multiome | 115 | |
| chr6:41,408,576–41,409,789 | 227.6 kb | Distal (>10kb) Multiome | 127 | |
| chr6:41,427,536–41,428,491 | 208.9 kb | Distal (>10kb) Multiome | 477 | |
| chr6:41,442,795–41,443,887 | 193.7 kb | Distal (>10kb) Multiome | 389 | |
| chr6:41,504,922–41,505,512 | 131.6 kb | Distal (>10kb) Multiome | 231 | |
| chr6:41,545,684–41,547,717 | 90.6 kb | Distal (>10kb) Multiome | 837 | |
| chr6:41,560,291–41,561,295 | 76.1 kb | Distal (>10kb) Multiome | 321 | |
| chr6:41,577,904–41,579,491 | 58.2 kb | Distal (>10kb) Multiome | 740 | |
| chr6:41,636,863–41,638,034 | at TSS | At TSS | 205 | |
| chr6:41,638,107–41,639,488 | 1.6 kb | Proximal (<10kb) Multiome | 240 | |
| chr6:41,644,322–41,644,671 | 7.4 kb | Proximal (<10kb) | 113 | |
| chr6:41,683,281–41,684,037 | 46.9 kb | Distal (>10kb) Multiome | 492 | |
| chr6:41,733,652–41,736,107 | 97.4 kb | Distal (>10kb) Multiome | 908 | |
| chr6:41,779,509–41,781,116 | 143.2 kb | Distal (>10kb) Multiome | 857 | |
| chr6:41,786,100–41,787,779 | 149.6 kb | Distal (>10kb) Multiome | 1106 | |
| chr6:41,894,347–41,896,221 | 258.5 kb | Distal (>10kb) Multiome | 822 | |
| chr6:41,920,834–41,921,901 | 284.4 kb | Distal (>10kb) Multiome | 955 |
Genomic view of the MDFI locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.