Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation. [provided by RefSeq, Oct 2015]
Transcription factors with Perturb-seq knockdown data for MACROH2A2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MACROH2A2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MACROH2A2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:69,801,126–69,803,261 | 249.9 kb | Distal (>10kb) Multiome | 527 | |
| chr10:69,876,839–69,877,624 | 175.6 kb | Distal (>10kb) Multiome | 45 | |
| chr10:70,052,471–70,054,619 | 71 bp | At TSS Multiome | 577 | |
| chr10:70,054,998–70,055,223 | 2.2 kb | Proximal (<10kb) | 37 | |
| chr10:70,075,065–70,075,566 | 22.4 kb | Distal (>10kb) Multiome | 23 | |
| chr10:70,116,082–70,116,892 | 63.6 kb | Distal (>10kb) Multiome | 217 | |
| chr10:70,132,124–70,133,061 | 79.9 kb | Distal (>10kb) Multiome | 729 | |
| chr10:70,145,328–70,147,235 | 92.8 kb | Distal (>10kb) Multiome | 1006 | |
| chr10:70,169,902–70,171,034 | 117.8 kb | Distal (>10kb) Multiome | 777 | |
| chr10:70,232,204–70,234,260 | 180.8 kb | Distal (>10kb) Multiome | 949 |
Genomic view of the MACROH2A2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.