This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ISL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ISL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ISL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:51,225,283–51,226,726 | 157.2 kb | Distal (>10kb) Multiome | 392 | |
| chr5:51,377,141–51,379,761 | 3.7 kb | Proximal (<10kb) | 245 | |
| chr5:51,381,588–51,382,293 | 1.2 kb | Proximal (<10kb) | 89 | |
| chr5:51,382,501–51,384,199 | 92 bp | At TSS Multiome | 426 | |
| chr5:51,387,200–51,388,228 | 3.8 kb | Proximal (<10kb) | 209 | |
| chr5:51,388,444–51,390,546 | 6.0 kb | Proximal (<10kb) Multiome | 237 | |
| chr5:51,390,715–51,391,601 | 7.8 kb | Proximal (<10kb) Multiome | 146 | |
| chr5:51,392,697–51,393,166 | 9.2 kb | Proximal (<10kb) | 72 |
Genomic view of the ISL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.