Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
Transcription factors with Perturb-seq knockdown data for IDH1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IDH1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IDH1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:207,970,009–207,971,155 | 284.6 kb | Distal (>10kb) Multiome | 256 | |
| chr2:208,025,250–208,025,873 | 229.6 kb | Distal (>10kb) Multiome | 1046 | |
| chr2:208,112,378–208,113,769 | 141.9 kb | Distal (>10kb) Multiome HiCAR | 285 | |
| chr2:208,124,049–208,124,760 | 130.7 kb | Distal (>10kb) Multiome | 140 | |
| chr2:208,142,720–208,143,849 | 111.7 kb | Distal (>10kb) Multiome HiCAR | 333 | |
| chr2:208,215,744–208,216,551 | 39.0 kb | Distal (>10kb) Multiome | 25 | |
| chr2:208,253,738–208,255,989 | 883 bp | At TSS Multiome | 1085 | |
| chr2:208,265,281–208,266,835 | 11.1 kb | Distal (>10kb) Multiome | 1005 | |
| chr2:208,267,505–208,268,088 | 12.7 kb | Distal (>10kb) Multiome | 169 | |
| chr2:208,317,884–208,318,936 | 63.2 kb | Distal (>10kb) Multiome | 106 | |
| chr2:208,406,386–208,407,453 | 151.8 kb | Distal (>10kb) Multiome | 324 |
Genomic view of the IDH1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.