Transcription factors with Perturb-seq knockdown data for HPAT5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HPAT5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HPAT5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:166,949,735–166,950,229 | 278.3 kb | Distal (>10kb) Multiome | 130 | |
| chr6:166,955,870–166,957,689 | 271.7 kb | Distal (>10kb) Multiome | 930 | |
| chr6:166,997,827–166,999,955 | 229.0 kb | Distal (>10kb) Multiome | 929 | |
| chr6:167,146,776–167,147,442 | 81.1 kb | Distal (>10kb) Multiome | 130 | |
| chr6:167,163,221–167,164,006 | 64.6 kb | Distal (>10kb) Multiome | 240 | |
| chr6:167,223,835–167,224,490 | 3.8 kb | Proximal (<10kb) | 385 | |
| chr6:167,225,871–167,226,407 | 1.9 kb | Proximal (<10kb) | 70 | |
| chr6:167,227,814–167,228,643 | 109 bp | At TSS Multiome | 140 | |
| chr6:167,236,265–167,236,542 | 8.0 kb | Proximal (<10kb) | 100 | |
| chr6:167,244,852–167,245,449 | 16.9 kb | Distal (>10kb) Multiome | 56 | |
| chr6:167,350,830–167,351,688 | 123.1 kb | Distal (>10kb) Multiome | 442 |
Genomic view of the HPAT5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.