This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]
Transcription factors with Perturb-seq knockdown data for GADD45A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GADD45A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GADD45A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:67,392,714–67,393,694 | 291.9 kb | Distal (>10kb) Multiome | 147 | |
| chr1:67,429,433–67,431,336 | 254.7 kb | Distal (>10kb) Multiome | 1000 | |
| chr1:67,458,750–67,459,497 | 226.2 kb | Distal (>10kb) Multiome | 199 | |
| chr1:67,500,441–67,500,998 | 184.6 kb | Distal (>10kb) Multiome | 516 | |
| chr1:67,515,399–67,516,305 | 169.4 kb | Distal (>10kb) Multiome | 174 | |
| chr1:67,539,374–67,540,683 | 144.9 kb | Distal (>10kb) Multiome HiCAR | 237 | |
| chr1:67,684,310–67,687,012 | 1.5 kb | Proximal (<10kb) Multiome | 1205 | |
| chr1:67,688,423–67,689,311 | 3.6 kb | Proximal (<10kb) Multiome | 464 | |
| chr1:67,689,796–67,690,191 | 4.6 kb | Proximal (<10kb) | 112 | |
| chr1:67,691,577–67,692,105 | 6.3 kb | Proximal (<10kb) | 99 | |
| chr1:67,692,869–67,693,238 | 7.6 kb | Proximal (<10kb) | 21 | |
| chr1:67,694,978–67,695,442 | 9.8 kb | Proximal (<10kb) | 166 | |
| chr1:67,749,369–67,750,469 | 64.5 kb | Distal (>10kb) Multiome HiCAR | 483 | |
| chr1:67,832,601–67,833,942 | 148.1 kb | Distal (>10kb) Multiome | 519 |
Genomic view of the GADD45A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.