This gene is a member of the F-box/WD-40 gene family, which recruit specific target proteins through their WD-40 protein-protein binding domains for ubiquitin mediated degradation. In mouse, a highly similar protein is thought to be responsible for maintaining the apical ectodermal ridge of developing limb buds; disruption of the mouse gene results in the absence of central digits, underdeveloped or absent metacarpal/metatarsal bones and syndactyly. This phenotype is remarkably similar to split hand-split foot malformation in humans, a clinically heterogeneous condition with a variety of modes of transmission. An autosomal recessive form has been mapped to the chromosomal region where this gene is located, and complex rearrangements involving duplications of this gene and others have been associated with the condition. A pseudogene of this locus has been mapped to one of the introns of the BCR gene on chromosome 22. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for FBXW4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FBXW4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FBXW4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:101,450,379–101,450,896 | 244.6 kb | Distal (>10kb) Multiome | 226 | |
| chr10:101,569,467–101,571,017 | 124.9 kb | Distal (>10kb) Multiome | 558 | |
| chr10:101,587,742–101,588,624 | 106.9 kb | Distal (>10kb) Multiome | 673 | |
| chr10:101,602,717–101,603,282 | 92.2 kb | Distal (>10kb) Multiome HiCAR | 348 | |
| chr10:101,606,618–101,607,814 | 87.9 kb | Distal (>10kb) Multiome HiCAR | 143 | |
| chr10:101,667,996–101,669,307 | 26.5 kb | Distal (>10kb) Multiome | 135 | |
| chr10:101,694,020–101,695,446 | 796 bp | At TSS Multiome | 821 | |
| chr10:101,749,494–101,750,047 | 54.6 kb | Distal (>10kb) Multiome | 178 | |
| chr10:101,775,454–101,777,009 | 81.3 kb | Distal (>10kb) Multiome | 202 | |
| chr10:101,778,233–101,781,743 | 86.0 kb | Distal (>10kb) Multiome | 720 | |
| chr10:101,782,912–101,783,976 | 88.3 kb | Distal (>10kb) Multiome | 661 | |
| chr10:101,817,350–101,819,352 | 123.4 kb | Distal (>10kb) Multiome | 1004 | |
| chr10:101,828,926–101,831,154 | 135.7 kb | Distal (>10kb) Multiome | 697 | |
| chr10:101,839,408–101,839,998 | 144.5 kb | Distal (>10kb) Multiome | 196 | |
| chr10:101,840,663–101,841,471 | 145.9 kb | Distal (>10kb) Multiome | 499 | |
| chr10:101,843,178–101,844,328 | 148.6 kb | Distal (>10kb) Multiome | 277 | |
| chr10:101,893,696–101,895,001 | 199.3 kb | Distal (>10kb) Multiome | 142 | |
| chr10:101,960,808–101,961,342 | 266.0 kb | Distal (>10kb) Multiome | 47 |
Genomic view of the FBXW4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.