Transcription factors with Perturb-seq knockdown data for FAM124A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FAM124A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FAM124A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr13:51,101,590–51,102,215 | 120.6 kb | Distal (>10kb) Multiome | 123 | |
| chr13:51,122,652–51,123,430 | 99.3 kb | Distal (>10kb) Multiome | 298 | |
| chr13:51,125,567–51,126,048 | 96.6 kb | Distal (>10kb) Multiome | 224 | |
| chr13:51,146,222–51,147,378 | 75.6 kb | Distal (>10kb) Multiome HiCAR | 79 | |
| chr13:51,147,892–51,149,246 | 73.9 kb | Distal (>10kb) Multiome HiCAR | 319 | |
| chr13:51,169,207–51,170,289 | 52.5 kb | Distal (>10kb) Multiome HiCAR | 174 | |
| chr13:51,221,124–51,223,194 | 231 bp | At TSS Multiome | 488 | |
| chr13:51,223,347–51,223,788 | 977 bp | At TSS | 51 | |
| chr13:51,229,803–51,230,043 | 7.4 kb | Proximal (<10kb) | 24 | |
| chr13:51,230,351–51,230,956 | 8.0 kb | Proximal (<10kb) | 66 | |
| chr13:51,243,717–51,244,186 | 21.5 kb | Distal (>10kb) Multiome | 185 | |
| chr13:51,244,496–51,245,455 | 22.9 kb | Distal (>10kb) Multiome | 117 | |
| chr13:51,303,027–51,303,676 | 80.9 kb | Distal (>10kb) Multiome | 130 | |
| chr13:51,451,249–51,454,202 | 229.4 kb | Distal (>10kb) Multiome | 1037 | |
| chr13:51,795,081–51,795,749 | 573.1 kb | Distal (>10kb) Multiome HiCAR | 50 |
Genomic view of the FAM124A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.