This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
Transcription factors with Perturb-seq knockdown data for EPHB2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EPHB2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EPHB2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:22,442,329–22,443,072 | 268.2 kb | Distal (>10kb) Multiome | 41 | |
| chr1:22,443,217–22,443,731 | 267.2 kb | Distal (>10kb) Multiome | 29 | |
| chr1:22,451,358–22,452,630 | 259.0 kb | Distal (>10kb) Multiome | 1027 | |
| chr1:22,704,537–22,704,942 | 5.9 kb | Proximal (<10kb) | 217 | |
| chr1:22,710,203–22,711,431 | 135 bp | At TSS Multiome | 253 | |
| chr1:22,716,768–22,717,592 | 6.4 kb | Proximal (<10kb) Multiome | 165 | |
| chr1:22,741,075–22,741,547 | 30.5 kb | Distal (>10kb) Multiome | 33 | |
| chr1:22,976,445–22,976,936 | 265.8 kb | Distal (>10kb) Multiome | 351 |
Genomic view of the EPHB2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.