Transcription factors with Perturb-seq knockdown data for EGILA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EGILA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EGILA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:20,412,688–20,413,758 | 293.8 kb | Distal (>10kb) Multiome | 865 | |
| chr14:20,454,384–20,455,825 | 252.0 kb | Distal (>10kb) Multiome | 1012 | |
| chr14:20,460,777–20,462,259 | 245.5 kb | Distal (>10kb) Multiome | 995 | |
| chr14:20,468,846–20,470,856 | 237.7 kb | Distal (>10kb) Multiome | 950 | |
| chr14:20,494,584–20,495,486 | 212.1 kb | Distal (>10kb) Multiome | 201 | |
| chr14:20,609,147–20,610,547 | 97.0 kb | Distal (>10kb) Multiome | 852 | |
| chr14:20,612,915–20,614,464 | 93.5 kb | Distal (>10kb) Multiome | 1003 | |
| chr14:20,624,684–20,626,122 | 81.7 kb | Distal (>10kb) Multiome | 728 | |
| chr14:20,630,325–20,631,286 | 76.4 kb | Distal (>10kb) Multiome | 507 | |
| chr14:20,631,700–20,633,862 | 73.9 kb | Distal (>10kb) Multiome | 757 | |
| chr14:20,642,696–20,643,499 | 64.1 kb | Distal (>10kb) Multiome | 52 | |
| chr14:20,644,459–20,645,301 | 62.3 kb | Distal (>10kb) Multiome | 52 | |
| chr14:20,652,905–20,654,391 | 53.8 kb | Distal (>10kb) Multiome | 320 | |
| chr14:20,657,425–20,658,132 | 49.3 kb | Distal (>10kb) Multiome | 320 | |
| chr14:20,662,835–20,663,940 | 43.8 kb | Distal (>10kb) Multiome | 587 | |
| chr14:20,681,216–20,685,294 | 22.7 kb | Distal (>10kb) Multiome | 1181 | |
| chr14:20,697,813–20,698,003 | 9.1 kb | Proximal (<10kb) | 171 | |
| chr14:20,702,153–20,702,358 | 4.8 kb | Proximal (<10kb) | 2 | |
| chr14:20,705,982–20,707,066 | 724 bp | At TSS Multiome | 110 | |
| chr14:20,711,665–20,712,029 | 4.5 kb | Proximal (<10kb) | 73 | |
| chr14:20,732,350–20,732,945 | 25.5 kb | Distal (>10kb) Multiome | 51 | |
| chr14:20,815,630–20,816,752 | 109.0 kb | Distal (>10kb) Multiome HiCAR | 75 | |
| chr14:20,820,324–20,820,826 | 113.5 kb | Distal (>10kb) Multiome | 204 | |
| chr14:20,970,757–20,971,991 | 264.3 kb | Distal (>10kb) Multiome | 745 | |
| chr14:20,989,595–20,990,562 | 282.8 kb | Distal (>10kb) Multiome | 835 |
Genomic view of the EGILA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.