The protein encoded by this gene is a member of the CAS (CRK-associated substrate) family of adaptor proteins which typically serve as scaffolds for the assembly of larger signaling complexes. These complexes form at the cell surface where integrin binding leads to the subsequent phosphorylation of a CAS protein. Additional binding of SRC family kinases leads to CAS hyperphosphorylation and the creation of binding sites for CRK and other proteins that cause actin cytoskeleton reorganization. This gene plays a role in integrin-mediated cell attachment, spreading, and migration and also plays a role in both normal and malignant cellular transformation. This broadly expressed gene has been shown to play a role in neurite outgrowth and its expression in the thymus and lymphocytes is important for T cell maturation and the development of immunological self-tolerance. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]
Transcription factors with Perturb-seq knockdown data for EFS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EFS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EFS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:23,101,326–23,101,861 | 264.0 kb | Distal (>10kb) Multiome | 83 | |
| chr14:23,154,159–23,154,639 | 211.2 kb | Distal (>10kb) Multiome | 392 | |
| chr14:23,161,040–23,161,530 | 204.3 kb | Distal (>10kb) Multiome | 397 | |
| chr14:23,182,495–23,182,942 | 182.9 kb | Distal (>10kb) Multiome | 139 | |
| chr14:23,237,732–23,238,192 | 127.7 kb | Distal (>10kb) Multiome | 536 | |
| chr14:23,285,641–23,286,830 | 79.4 kb | Distal (>10kb) Multiome | 962 | |
| chr14:23,300,944–23,303,002 | 64.0 kb | Distal (>10kb) Multiome | 1096 | |
| chr14:23,306,324–23,307,321 | 58.9 kb | Distal (>10kb) Multiome | 871 | |
| chr14:23,320,558–23,322,196 | 44.4 kb | Distal (>10kb) Multiome | 1090 | |
| chr14:23,351,817–23,353,063 | 12.8 kb | Distal (>10kb) Multiome | 403 | |
| chr14:23,358,162–23,358,640 | 6.5 kb | Proximal (<10kb) | 31 | |
| chr14:23,364,202–23,367,105 | 834 bp | At TSS Multiome | 353 | |
| chr14:23,468,966–23,469,987 | 103.8 kb | Distal (>10kb) Multiome | 955 | |
| chr14:23,493,984–23,494,589 | 128.7 kb | Distal (>10kb) Multiome | 131 | |
| chr14:23,537,253–23,538,372 | 171.9 kb | Distal (>10kb) Multiome | 160 | |
| chr14:23,544,507–23,545,444 | 179.1 kb | Distal (>10kb) Multiome | 308 | |
| chr14:23,550,782–23,552,258 | 186.1 kb | Distal (>10kb) Multiome | 826 | |
| chr14:23,555,640–23,556,485 | 190.5 kb | Distal (>10kb) Multiome | 983 | |
| chr14:23,567,065–23,567,976 | 202.1 kb | Distal (>10kb) Multiome | 855 | |
| chr14:23,575,776–23,576,501 | 210.6 kb | Distal (>10kb) Multiome | 163 | |
| chr14:23,589,018–23,589,481 | 223.6 kb | Distal (>10kb) Multiome | 770 | |
| chr14:23,631,551–23,632,458 | 266.5 kb | Distal (>10kb) Multiome | 545 |
Genomic view of the EFS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.