Transcription factors with Perturb-seq knockdown data for EEIG2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EEIG2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EEIG2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:108,533,494–108,534,081 | 26.3 kb | Distal (>10kb) Multiome | 360 | |
| chr1:108,559,314–108,561,269 | 58 bp | At TSS Multiome | 794 | |
| chr1:108,561,781–108,562,230 | 1.7 kb | Proximal (<10kb) | 337 | |
| chr1:108,660,822–108,661,680 | 101.2 kb | Distal (>10kb) Multiome | 460 | |
| chr1:108,685,537–108,686,147 | 125.8 kb | Distal (>10kb) Multiome | 57 | |
| chr1:108,691,720–108,693,647 | 132.3 kb | Distal (>10kb) Multiome | 985 | |
| chr1:108,746,190–108,747,539 | 186.6 kb | Distal (>10kb) Multiome | 1013 | |
| chr1:108,828,188–108,828,798 | 268.3 kb | Distal (>10kb) Multiome | 359 | |
| chr1:108,842,454–108,843,162 | 282.6 kb | Distal (>10kb) Multiome | 142 | |
| chr1:108,845,269–108,845,935 | 285.5 kb | Distal (>10kb) Multiome | 30 |
Genomic view of the EEIG2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.