The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]
Transcription factors with Perturb-seq knockdown data for DUSP6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DUSP6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DUSP6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:89,098,193–89,099,140 | 253.6 kb | Distal (>10kb) Multiome HiCAR | 188 | |
| chr12:89,225,440–89,226,374 | 126.4 kb | Distal (>10kb) Multiome HiCAR | 416 | |
| chr12:89,247,539–89,248,148 | 104.6 kb | Distal (>10kb) Multiome HiCAR | 291 | |
| chr12:89,344,903–89,347,295 | 6.6 kb | Proximal (<10kb) Multiome | 690 | |
| chr12:89,349,881–89,350,937 | 2.2 kb | Proximal (<10kb) Multiome | 360 | |
| chr12:89,351,407–89,355,035 | 1.5 kb | Proximal (<10kb) Multiome | 1222 | |
| chr12:89,355,213–89,355,578 | 2.8 kb | Proximal (<10kb) | 141 | |
| chr12:89,357,268–89,357,606 | 4.9 kb | Proximal (<10kb) | 262 | |
| chr12:89,411,690–89,412,513 | 59.7 kb | Distal (>10kb) Multiome HiCAR | 89 | |
| chr12:89,524,103–89,526,498 | 173.6 kb | Distal (>10kb) Multiome HiCAR | 1007 |
Genomic view of the DUSP6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.