DNAJA1
DnaJ heat shock protein family (Hsp40) member A1 | HSPF4, NEDD7, dj-2, hdj-2, HSJ2

This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes. [provided by RefSeq, Sep 2015]

Member of: DE-1 DE-1.7 Developmental clusters: GC5
Biological processes 44 terms
ATP binding (GO:0005524)ATPase activator activity (GO:0001671)ATPase activator activity (GO:0001671)ATPase activator activity (GO:0001671)C3HC4-type RING finger domain binding (GO:0055131)G protein-coupled receptor binding (GO:0001664)Hsp70 protein binding (GO:0030544)Hsp70 protein binding (GO:0030544)Tat protein binding (GO:0030957)cytoplasm (GO:0005737)cytoplasmic side of endoplasmic reticulum membrane (GO:0098554)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)extracellular exosome (GO:0070062)heat shock protein binding (GO:0031072)low-density lipoprotein particle receptor binding (GO:0050750)membrane (GO:0016020)membrane (GO:0016020)membrane (GO:0016020)microtubule cytoskeleton (GO:0015630)mitochondrion (GO:0005739)negative regulation of JUN kinase activity (GO:0043508)negative regulation of apoptotic process (GO:0043066)negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway (GO:1905259)negative regulation of protein localization to mitochondrion (GO:1903748)negative regulation of protein ubiquitination (GO:0031397)nucleus (GO:0005634)perinuclear region of cytoplasm (GO:0048471)positive regulation of apoptotic process (GO:0043065)protein binding (GO:0005515)protein folding (GO:0006457)protein folding (GO:0006457)protein localization to mitochondrion (GO:0070585)protein localization to mitochondrion (GO:0070585)protein-folding chaperone binding (GO:0051087)protein-folding chaperone binding (GO:0051087)protein-folding chaperone binding (GO:0051087)protein-folding chaperone binding (GO:0051087)regulation of protein transport (GO:0051223)response to heat (GO:0009408)response to unfolded protein (GO:0006986)ubiquitin protein ligase binding (GO:0031625)unfolded protein binding (GO:0051082)
Expression (TPM)
DNAJA1 — as a Regulated Gene

TFs regulating DNAJA1 0 TFs

Transcription factors with Perturb-seq knockdown data for DNAJA1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DNAJA1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to DNAJA1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DNAJA1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr9:32,782,490–32,784,047 241.8 kb Distal (>10kb) Multiome 305
chr9:32,950,445–32,951,255 74.4 kb Distal (>10kb) Multiome 12
chr9:33,000,845–33,002,021 23.7 kb Distal (>10kb) Multiome 802
chr9:33,024,523–33,026,243 26 bp At TSS Multiome 972
chr9:33,044,108–33,044,981 19.3 kb Distal (>10kb) Multiome 451
chr9:33,076,221–33,076,965 51.5 kb Distal (>10kb) Multiome 804
chr9:33,166,320–33,168,338 142.1 kb Distal (>10kb) Multiome 849
chr9:33,171,750–33,173,178 147.3 kb Distal (>10kb) Multiome 359
chr9:33,264,068–33,265,475 239.5 kb Distal (>10kb) Multiome 790
chr9:33,267,199–33,267,770 242.2 kb Distal (>10kb) Multiome 106
chr9:33,290,056–33,291,152 265.2 kb Distal (>10kb) Multiome 1075

Genome Browser

Genomic view of the DNAJA1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr9:32,772,490 – 33,301,152
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq