This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for CKAP5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CKAP5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CKAP5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:46,554,280–46,555,184 | 291.6 kb | Distal (>10kb) Multiome | 531 | |
| chr11:46,593,562–46,594,345 | 252.3 kb | Distal (>10kb) Multiome | 845 | |
| chr11:46,616,855–46,618,064 | 229.0 kb | Distal (>10kb) Multiome | 823 | |
| chr11:46,664,502–46,665,016 | 181.5 kb | Distal (>10kb) Multiome | 126 | |
| chr11:46,674,791–46,675,556 | 171.2 kb | Distal (>10kb) Multiome | 308 | |
| chr11:46,700,262–46,701,275 | 145.6 kb | Distal (>10kb) Multiome | 997 | |
| chr11:46,826,562–46,827,053 | 19.4 kb | Distal (>10kb) Multiome | 529 | |
| chr11:46,845,538–46,846,680 | 55 bp | At TSS Multiome | 879 | |
| chr11:46,912,540–46,913,271 | 66.6 kb | Distal (>10kb) Multiome | 252 | |
| chr11:46,917,991–46,919,090 | 72.3 kb | Distal (>10kb) Multiome | 405 | |
| chr11:46,936,572–46,937,187 | 90.5 kb | Distal (>10kb) Multiome | 744 | |
| chr11:46,947,422–46,947,958 | 101.5 kb | Distal (>10kb) Multiome | 150 |
Genomic view of the CKAP5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.