CHEK1

checkpoint kinase 1 | CHK1

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Member of: DE-6 DE-6.1 Developmental clusters: GC5

Biological processes 57 terms

ATP binding (GO:0005524)DNA damage checkpoint signaling (GO:0000077)DNA damage checkpoint signaling (GO:0000077)DNA damage checkpoint signaling (GO:0000077)DNA damage response (GO:0006974)DNA damage response (GO:0006974)DNA repair (GO:0006281)DNA replication (GO:0006260)cellular response to mechanical stimulus (GO:0071260)centrosome (GO:0005813)centrosome (GO:0005813)chromatin (GO:0000785)chromatin (GO:0000785)chromatin remodeling (GO:0006338)chromatin remodeling (GO:0006338)chromosome (GO:0005694)chromosome, telomeric region (GO:0000781)condensed nuclear chromosome (GO:0000794)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)extracellular region (GO:0005576)histone H3T11 kinase activity (GO:0035402)histone H3T11 kinase activity (GO:0035402)histone H3T11 kinase activity (GO:0035402)mitotic G2 DNA damage checkpoint signaling (GO:0007095)mitotic G2 DNA damage checkpoint signaling (GO:0007095)mitotic G2/M transition checkpoint (GO:0044818)mitotic G2/M transition checkpoint (GO:0044818)negative regulation of G0 to G1 transition (GO:0070317)negative regulation of cell cycle phase transition (GO:1901988)negative regulation of gene expression, epigenetic (GO:0045814)negative regulation of gene expression, epigenetic (GO:0045814)negative regulation of mitotic nuclear division (GO:0045839)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)peptidyl-threonine phosphorylation (GO:0018107)positive regulation of cell cycle (GO:0045787)protein binding (GO:0005515)protein domain specific binding (GO:0019904)protein kinase activity (GO:0004672)protein kinase activity (GO:0004672)protein phosphorylation (GO:0006468)protein serine kinase activity (GO:0106310)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein-containing complex (GO:0032991)regulation of double-strand break repair via homologous recombination (GO:0010569)regulation of mitotic centrosome separation (GO:0046602)regulation of signal transduction by p53 class mediator (GO:1901796)replication fork (GO:0005657)replicative senescence (GO:0090399)signal transduction in response to DNA damage (GO:0042770)signal transduction in response to DNA damage (GO:0042770)

Expression (TPM)

TFs regulating CHEK1 0 TFs

Transcription factors with Perturb-seq knockdown data for CHEK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHEK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to CHEK1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHEK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr11:125,330,306–125,331,087	295.2 kb	Distal (>10kb) Multiome	104
chr11:125,369,857–125,372,110	255.7 kb	Distal (>10kb) Multiome	226
chr11:125,404,667–125,405,169	221.0 kb	Distal (>10kb) Multiome	273
chr11:125,433,337–125,433,944	192.3 kb	Distal (>10kb) Multiome	170
chr11:125,443,557–125,444,361	182.0 kb	Distal (>10kb) Multiome	142
chr11:125,495,125–125,496,641	129.7 kb	Distal (>10kb) Multiome	422
chr11:125,505,583–125,506,532	120.0 kb	Distal (>10kb) Multiome	40
chr11:125,509,289–125,509,972	116.4 kb	Distal (>10kb) Multiome	165
chr11:125,568,984–125,569,972	56.6 kb	Distal (>10kb) Multiome	796
chr11:125,591,812–125,593,372	33.3 kb	Distal (>10kb) Multiome	902
chr11:125,625,323–125,626,856	108 bp	At TSS Multiome	1108
chr11:125,886,970–125,888,478	261.8 kb	Distal (>10kb) Multiome	823
chr11:125,902,922–125,903,413	277.2 kb	Distal (>10kb) Multiome	495
chr11:125,904,107–125,904,760	278.5 kb	Distal (>10kb) Multiome	235

Genome Browser

Genomic view of the CHEK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.