Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CHAF1B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHAF1B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHAF1B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr21:36,096,914–36,097,455 | 288.2 kb | Distal (>10kb) Multiome | 76 | |
| chr21:36,099,712–36,100,295 | 285.4 kb | Distal (>10kb) Multiome | 79 | |
| chr21:36,104,387–36,104,959 | 280.6 kb | Distal (>10kb) Multiome | 137 | |
| chr21:36,155,542–36,157,554 | 228.8 kb | Distal (>10kb) Multiome | 789 | |
| chr21:36,319,638–36,321,035 | 65.2 kb | Distal (>10kb) Multiome | 945 | |
| chr21:36,385,010–36,385,875 | 10 bp | At TSS Multiome | 530 | |
| chr21:36,422,324–36,422,828 | 37.1 kb | Distal (>10kb) Multiome | 259 | |
| chr21:36,429,831–36,430,388 | 44.8 kb | Distal (>10kb) Multiome | 600 | |
| chr21:36,434,206–36,435,196 | 49.3 kb | Distal (>10kb) Multiome | 69 |
Genomic view of the CHAF1B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.