This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for BUB3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BUB3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BUB3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:122,875,885–122,876,836 | 278.1 kb | Distal (>10kb) Multiome | 128 | |
| chr10:122,877,140–122,877,717 | 276.9 kb | Distal (>10kb) Multiome | 112 | |
| chr10:122,879,124–122,880,264 | 274.6 kb | Distal (>10kb) Multiome | 712 | |
| chr10:122,953,726–122,954,686 | 200.1 kb | Distal (>10kb) Multiome | 995 | |
| chr10:122,980,093–122,981,015 | 173.9 kb | Distal (>10kb) Multiome | 874 | |
| chr10:123,007,898–123,009,768 | 145.5 kb | Distal (>10kb) Multiome | 911 | |
| chr10:123,132,736–123,133,704 | 21.2 kb | Distal (>10kb) Multiome | 384 | |
| chr10:123,135,104–123,137,885 | 17.1 kb | Distal (>10kb) Multiome | 563 | |
| chr10:123,139,520–123,140,375 | 14.4 kb | Distal (>10kb) Multiome | 456 | |
| chr10:123,144,827–123,145,179 | 9.2 kb | Proximal (<10kb) | 98 | |
| chr10:123,147,570–123,148,872 | 6.3 kb | Proximal (<10kb) Multiome | 310 | |
| chr10:123,149,449–123,150,210 | 4.2 kb | Proximal (<10kb) | 125 | |
| chr10:123,150,788–123,151,726 | 3.2 kb | Proximal (<10kb) Multiome | 224 | |
| chr10:123,153,975–123,155,184 | 121 bp | At TSS Multiome | 787 |
Genomic view of the BUB3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.