Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ARHGEF3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARHGEF3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARHGEF3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:56,782,366–56,782,841 | 296.6 kb | Distal (>10kb) Multiome | 265 | |
| chr3:56,801,406–56,802,556 | 277.2 kb | Distal (>10kb) Multiome | 717 | |
| chr3:56,823,361–56,824,198 | 255.5 kb | Distal (>10kb) Multiome | 160 | |
| chr3:56,874,031–56,874,571 | 204.9 kb | Distal (>10kb) Multiome | 83 | |
| chr3:57,078,355–57,079,870 | 8 bp | At TSS Multiome | 674 | |
| chr3:57,130,457–57,131,979 | 51.7 kb | Distal (>10kb) Multiome | 353 | |
| chr3:57,158,954–57,159,929 | 80.1 kb | Distal (>10kb) Multiome | 259 | |
| chr3:57,160,383–57,161,092 | 81.5 kb | Distal (>10kb) Multiome | 147 | |
| chr3:57,164,404–57,166,039 | 86.1 kb | Distal (>10kb) Multiome | 455 | |
| chr3:57,169,750–57,170,529 | 91.0 kb | Distal (>10kb) Multiome | 491 | |
| chr3:57,194,399–57,195,064 | 115.5 kb | Distal (>10kb) Multiome | 31 | |
| chr3:57,199,761–57,200,263 | 120.8 kb | Distal (>10kb) Multiome | 256 | |
| chr3:57,227,073–57,228,173 | 148.4 kb | Distal (>10kb) Multiome | 859 |
Genomic view of the ARHGEF3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.